Characterization of Flanking Sequences Using Long Inverse PCR
نویسندگان
چکیده
منابع مشابه
Modified inverse PCR method for cloning the flanking sequences from human cell pools.
Inverse polymerase chain reaction (PCR) is a powerful approach for cloning the DNA sequences lying outside the boundaries of known sequences (7). This approach can be used to determine the integration site of a virus or a transposon (2,4). The procedure described by Triglia (7) can be directly used to amplify the flanking sequence in a plasmid or in a bacterial genome. However, it has been diff...
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Microsatellite polymorphisms are widely being employed as a major tool in genome analysis. Analysis of length polymorphisms in the PCR depends on the elucidation of the sequence of their flanking regions. Different methods have been developed for the efficient characterization of the flanking sequences. Small insert genomic libraries directly suitable for sequencing of the entire insert and hig...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00285bm03